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rabbit polyclonal anticofilin antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit polyclonal anticofilin antibody
    nArgBP2 KD increases WAVE1/PAK/cofilin phosphorylation, and counteracting this cascade rescues nArgBP2 KD effects on spines. Cortical neurons infected with AAV-scrambled or shRNA-nArgBP2 at DIV6, lysed at DIV21, and immunoblotted with anti-WAVE1 and phospho-WAVE1 antibody (A), PAK and phospho-PAK antibody (B), and <t>anticofilin</t> and phosphocofilin antibody (C) are shown. IB, immunoblot. (D) Representative images of spine from dendrites of neurons expressing each set of constructs. Hippocampal neurons were transfected at DIV9 and fixed at DIV16. Control, scrambled; shRNA (sh), shRNA-nArgBP2; S3A, phosphodeficient mutant of cofilin; mSH3-1/2, first and second SH3 domains of nArgBP2 targeted to mitochondria; mSH3-3, third SH3 domain of nArgBP2 targeted mitochondria. (Lower) Enlarged images of the insets enclosed with rectangles. (Scale bars: Upper, 10 μm; Lower, 2 μm.) The numbers of mushroom-shaped spines (E and G) and filopodia (F and H) from each experimental group are shown. *P < 0.05 by ANOVA, followed by Tukey’s honest significant difference (HSD) post hoc test [n = 45 neurons from seven independent cultures (E and F) and n = 39 neurons from four independent cultures (G and H)].
    Rabbit Polyclonal Anticofilin Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anticofilin/anti+phospho+cofilin/pmc04914163-338-0-9
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal anticofilin antibody - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "nArgBP2 regulates excitatory synapse formation by controlling dendritic spine morphology"

    Article Title: nArgBP2 regulates excitatory synapse formation by controlling dendritic spine morphology

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.1600944113

    nArgBP2 KD increases WAVE1/PAK/cofilin phosphorylation, and counteracting this cascade rescues nArgBP2 KD effects on spines. Cortical neurons infected with AAV-scrambled or shRNA-nArgBP2 at DIV6, lysed at DIV21, and immunoblotted with anti-WAVE1 and phospho-WAVE1 antibody (A), PAK and phospho-PAK antibody (B), and anticofilin and phosphocofilin antibody (C) are shown. IB, immunoblot. (D) Representative images of spine from dendrites of neurons expressing each set of constructs. Hippocampal neurons were transfected at DIV9 and fixed at DIV16. Control, scrambled; shRNA (sh), shRNA-nArgBP2; S3A, phosphodeficient mutant of cofilin; mSH3-1/2, first and second SH3 domains of nArgBP2 targeted to mitochondria; mSH3-3, third SH3 domain of nArgBP2 targeted mitochondria. (Lower) Enlarged images of the insets enclosed with rectangles. (Scale bars: Upper, 10 μm; Lower, 2 μm.) The numbers of mushroom-shaped spines (E and G) and filopodia (F and H) from each experimental group are shown. *P < 0.05 by ANOVA, followed by Tukey’s honest significant difference (HSD) post hoc test [n = 45 neurons from seven independent cultures (E and F) and n = 39 neurons from four independent cultures (G and H)].
    Figure Legend Snippet: nArgBP2 KD increases WAVE1/PAK/cofilin phosphorylation, and counteracting this cascade rescues nArgBP2 KD effects on spines. Cortical neurons infected with AAV-scrambled or shRNA-nArgBP2 at DIV6, lysed at DIV21, and immunoblotted with anti-WAVE1 and phospho-WAVE1 antibody (A), PAK and phospho-PAK antibody (B), and anticofilin and phosphocofilin antibody (C) are shown. IB, immunoblot. (D) Representative images of spine from dendrites of neurons expressing each set of constructs. Hippocampal neurons were transfected at DIV9 and fixed at DIV16. Control, scrambled; shRNA (sh), shRNA-nArgBP2; S3A, phosphodeficient mutant of cofilin; mSH3-1/2, first and second SH3 domains of nArgBP2 targeted to mitochondria; mSH3-3, third SH3 domain of nArgBP2 targeted mitochondria. (Lower) Enlarged images of the insets enclosed with rectangles. (Scale bars: Upper, 10 μm; Lower, 2 μm.) The numbers of mushroom-shaped spines (E and G) and filopodia (F and H) from each experimental group are shown. *P < 0.05 by ANOVA, followed by Tukey’s honest significant difference (HSD) post hoc test [n = 45 neurons from seven independent cultures (E and F) and n = 39 neurons from four independent cultures (G and H)].

    Techniques Used: Infection, shRNA, Western Blot, Expressing, Construct, Transfection, Mutagenesis

    Related Articles

    Phospho-proteomics:

    Article Title: Rho-associated kinase plays a role in rabbit urethral smooth muscle contraction, but not via enhanced myosin light chain phosphorylation.
    Article Snippet: .. Commercial antibodies were purchased from the following sources: rabbit polyclonal anti-LC20 (Santa Cruz Biotechnology), raised against the full-length human protein; rabbit polyclonal anti-pS19LC20 (Rockland), raised against a synthetic phosphopeptide corresponding to the region of the human protein containing pS19; rabbit polyclonal anti-CPI-17 (Upstate), raised against full-length recombinant porcine CPI-17; rabbit polyclonal anti-actin (Cytoskeleton), raised against a synthetic peptide corresponding to the COOH-terminal 11 residues of human actin (SGPSIVHRKCF); goat polyclonal anti-SM-22 (Novus Biologicals), raised against a synthetic peptide (MTGYGRPRQIIS) corresponding to residues 189–200 of human SM-22 ; rabbit polyclonal anti-pT697-MYPT1 (Upstate), raised against a synthetic phosphopeptide corresponding to the region around pT697 of the human protein; rabbit polyclonal anti-pT855MYPT1 (Upstate), raised against a synthetic phosphopeptide corresponding to the region around pT855 of the human protein; rabbit polyclonal anti-pS3-cofilin (Cell Signaling Technology), raised against a synthetic phosphopeptide corresponding to human cofilin containing phosphoserine at position 3; and rabbit polyclonal anticofilin (Cell Signaling Technology), raised against a synthetic peptide corresponding to human cofilin containing serine at position 3. .. N - nitro-L-arginine, atropine, phenylephrine, and phorbol 12,13-dibutyrate (PdBu) were purchased from Sigma; Y27632 from BioMol International; H1152, GF109203X, wortmannin, and calyculin-A from Calbiochem; and microcystin-LR from Alexis Biochemicals.

    Recombinant:

    Article Title: Rho-associated kinase plays a role in rabbit urethral smooth muscle contraction, but not via enhanced myosin light chain phosphorylation.
    Article Snippet: .. Commercial antibodies were purchased from the following sources: rabbit polyclonal anti-LC20 (Santa Cruz Biotechnology), raised against the full-length human protein; rabbit polyclonal anti-pS19LC20 (Rockland), raised against a synthetic phosphopeptide corresponding to the region of the human protein containing pS19; rabbit polyclonal anti-CPI-17 (Upstate), raised against full-length recombinant porcine CPI-17; rabbit polyclonal anti-actin (Cytoskeleton), raised against a synthetic peptide corresponding to the COOH-terminal 11 residues of human actin (SGPSIVHRKCF); goat polyclonal anti-SM-22 (Novus Biologicals), raised against a synthetic peptide (MTGYGRPRQIIS) corresponding to residues 189–200 of human SM-22 ; rabbit polyclonal anti-pT697-MYPT1 (Upstate), raised against a synthetic phosphopeptide corresponding to the region around pT697 of the human protein; rabbit polyclonal anti-pT855MYPT1 (Upstate), raised against a synthetic phosphopeptide corresponding to the region around pT855 of the human protein; rabbit polyclonal anti-pS3-cofilin (Cell Signaling Technology), raised against a synthetic phosphopeptide corresponding to human cofilin containing phosphoserine at position 3; and rabbit polyclonal anticofilin (Cell Signaling Technology), raised against a synthetic peptide corresponding to human cofilin containing serine at position 3. .. N - nitro-L-arginine, atropine, phenylephrine, and phorbol 12,13-dibutyrate (PdBu) were purchased from Sigma; Y27632 from BioMol International; H1152, GF109203X, wortmannin, and calyculin-A from Calbiochem; and microcystin-LR from Alexis Biochemicals.



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    Image Search Results


    nArgBP2 KD increases WAVE1/PAK/cofilin phosphorylation, and counteracting this cascade rescues nArgBP2 KD effects on spines. Cortical neurons infected with AAV-scrambled or shRNA-nArgBP2 at DIV6, lysed at DIV21, and immunoblotted with anti-WAVE1 and phospho-WAVE1 antibody (A), PAK and phospho-PAK antibody (B), and anticofilin and phosphocofilin antibody (C) are shown. IB, immunoblot. (D) Representative images of spine from dendrites of neurons expressing each set of constructs. Hippocampal neurons were transfected at DIV9 and fixed at DIV16. Control, scrambled; shRNA (sh), shRNA-nArgBP2; S3A, phosphodeficient mutant of cofilin; mSH3-1/2, first and second SH3 domains of nArgBP2 targeted to mitochondria; mSH3-3, third SH3 domain of nArgBP2 targeted mitochondria. (Lower) Enlarged images of the insets enclosed with rectangles. (Scale bars: Upper, 10 μm; Lower, 2 μm.) The numbers of mushroom-shaped spines (E and G) and filopodia (F and H) from each experimental group are shown. *P < 0.05 by ANOVA, followed by Tukey’s honest significant difference (HSD) post hoc test [n = 45 neurons from seven independent cultures (E and F) and n = 39 neurons from four independent cultures (G and H)].

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: nArgBP2 regulates excitatory synapse formation by controlling dendritic spine morphology

    doi: 10.1073/pnas.1600944113

    Figure Lengend Snippet: nArgBP2 KD increases WAVE1/PAK/cofilin phosphorylation, and counteracting this cascade rescues nArgBP2 KD effects on spines. Cortical neurons infected with AAV-scrambled or shRNA-nArgBP2 at DIV6, lysed at DIV21, and immunoblotted with anti-WAVE1 and phospho-WAVE1 antibody (A), PAK and phospho-PAK antibody (B), and anticofilin and phosphocofilin antibody (C) are shown. IB, immunoblot. (D) Representative images of spine from dendrites of neurons expressing each set of constructs. Hippocampal neurons were transfected at DIV9 and fixed at DIV16. Control, scrambled; shRNA (sh), shRNA-nArgBP2; S3A, phosphodeficient mutant of cofilin; mSH3-1/2, first and second SH3 domains of nArgBP2 targeted to mitochondria; mSH3-3, third SH3 domain of nArgBP2 targeted mitochondria. (Lower) Enlarged images of the insets enclosed with rectangles. (Scale bars: Upper, 10 μm; Lower, 2 μm.) The numbers of mushroom-shaped spines (E and G) and filopodia (F and H) from each experimental group are shown. *P < 0.05 by ANOVA, followed by Tukey’s honest significant difference (HSD) post hoc test [n = 45 neurons from seven independent cultures (E and F) and n = 39 neurons from four independent cultures (G and H)].

    Article Snippet: Rabbit polyclonal anticofilin antibody and phosphocofilin antibody were from Cell Signaling Technology.

    Techniques: Infection, shRNA, Western Blot, Expressing, Construct, Transfection, Mutagenesis